Apr 15, 2022 2:43:00 PM | TCAP The Utility of TCAP

TriMetis Computer-Assisted Pathology, the Utility of TCAP, and the grading scale of FFPE's.

Good, Better, Best

Grading FFPE's

Over the past 20 years, we have asked our clients, how much tumor do you need?  

The answers were usually a minimum % tumor and maximum % necrosis, often referred to as tumor purity.

When we asked "Is there any way we can define tumor purity so its less subjective between pathologists?", our partners were usually drifted into thought, with no solution. 

We would work together to deconstruct the standard for tumor purity, but would land on "Well, I guess that's the way we've always done it!" 

Grades of Strawberries

Industries other than Oncology use different methods for determining the quality of a product.

The USDA has a system for scoring strawberries. 

  • U.S. No. 1 consists of strawberries of one variety or similar varietal characteristics with the cap (calyx) attached, which are firm, not overripe or undeveloped, and which are free from mold or decay and free from damage caused by dirt, moisture, foreign matter, disease, insects, or mechanical or other means. Each strawberry has not less than three-fourths of its surface showing a pink or red color.
    1. Size. Unless otherwise specified, the minimum diameter of each strawberry is not less than three-fourths inch.
    2. Tolerances. In order to allow for variations incident to proper grading and handling the following tolerances, by count, are provided as specified:
      1. For defects. Not more than 10 percent for strawberries in any lot which fail to meet the requirements of this grade, but not more than one-half of this tolerance, or 5 percent, shall be allowed for defects causing serious damage, including therein not more than two-fifths of this latter amount, or 2 percent, for strawberries affected by decay.
      2. For off-size. Not more than 5 percent for strawberries in any lot which are below the specified minimum size

Strawberry Grading


So which is best for my application?

Which strawberry is best for my application?

  • Chocolate Covered
  • Jams/Jelly
  • Pies
  • Preserves
  • Additives

FFPE Sample Grading-1

Which FFPE is best for my application?

  • Immunohistochemistry
  • Laser Capture
  • NextGen Sequencing
  • Spatial Genomics
Leif Honda

Written By: Leif Honda